Studies on non-specific interference due to serum in the avidin-biotin microELISA for monkey chorionic gonadotropin and a method for its elimination

Jagannadha Rao, A. ; Chakraborti, R. (1989) Studies on non-specific interference due to serum in the avidin-biotin microELISA for monkey chorionic gonadotropin and a method for its elimination Journal of Immunological Methods, 125 (1-2). pp. 261-264. ISSN 0022-1759

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/002217...

Related URL: http://dx.doi.org/10.1016/0022-1759(89)90101-4

Abstract

A study has been carried out on the non-specific interference due to serum in the avidin biotin microELISA for monkey chorionic gonadotropin. Results suggest that it is not due to any proteolytic activity in the serum, but immunoglobulin or associated factors interfering at the level of antigen-antibody interaction. This interference was eliminated by heating samples by heatingsamples at 60°C for 30 min.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:ELISA; γ-globulin; Bovine; Gonadotrophin
ID Code:43023
Deposited On:09 Jun 2011 08:47
Last Modified:09 Jun 2011 08:47

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