An avidin-biotin micro-enzymeimmunoassay for monkey chorionic gonadotrophin

Chakrabarti, R. ; Jagannadha Rao, A. (1987) An avidin-biotin micro-enzymeimmunoassay for monkey chorionic gonadotrophin Journal of Reproduction and Fertility, 80 . pp. 151-158. ISSN 0022-4251

PDF - Publisher Version

Official URL:

Related URL:


The avidin-biotin micro-enzymeimmunoassay used antiserum to the β-subunit of sheep LH, biotinylated hCG as tracer and pooled serum from pregnant monkeys as a reference preparation of monkey CG. The assay was validated for specificity, by checking serum samples known to contain high quantities of LH, and false positive results were eliminated to a great extent by prior heating of serum samples at 60°C for 30 min. In 45 bonnet monkeys the test gave 10 true positives, 34 true negatives and 1 false positive, i.e. an accuracy of 97%. The serum profiles of CG, measured by the EIA, in bonnet, cynomolgus and rhesus monkeys during early pregnancy were very similar to those reported for bonnet and rhesus monkeys when a liquid-phase radioimmunoassay was used.

Item Type:Article
Source:Copyright of this article belongs to Society for Reproduction and Fertility.
ID Code:43020
Deposited On:09 Jun 2011 08:36
Last Modified:18 May 2016 00:07

Repository Staff Only: item control page