Jagannadha Rao, A. ; Behrens, Catherine ; Ramachandran, J. (1980) Immunochemical studies of adrenocorticotropin using tritium labeled hormone International Journal of Peptide and Protein Research, 15 (5). pp. 480-484. ISSN 0367-8377
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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1399-...
Related URL: http://dx.doi.org/10.1111/j.1399-3011.1980.tb02925.x
Abstract
A radioimmunoassay using specifically tritiated corticotropin (3H-ACTH) of high specific radioactivity (46 Ci/mmol) and full biological activity has been developed. The assay is sensitive to ACTH in the range 200 pg-10 ng. 50% displacement of 3H-ACTH is produced by less than a twofold excess of unlabeled hormone. Immunochemical studies with various ACTH analogs showed that the antiserum recognized the intact molecule better than any of the fragments.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Munksgaard International Publishers. |
| Keywords: | ACTH Analogs; ACTH Antiserum; Radioimmunoassay; Tritiated ACTH |
| ID Code: | 43016 |
| Deposited On: | 09 Jun 2011 08:07 |
| Last Modified: | 09 Jun 2011 08:07 |
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