Studies on the induction of δ-aminolevulinic acid synthetase in mouse liver

Gayathri, A. K. ; Rao, M. R. S. ; Padmanaban, G. (1973) Studies on the induction of δ-aminolevulinic acid synthetase in mouse liver Archives of Biochemistry and Biophysics, 155 (2). pp. 299-306. ISSN 0003-9861

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Administration of 3,5-diethoxy carbonyl-1,4-dihydrocollidine (DDC) to mice resulted in a striking increase in the level of δ-aminolevulinic acid (ALA) synthetase in liver. Although the enzyme activity was primarily localized in mitochondria and postmicrosomal supernatant fluid, a significant level of activity was also detected in purified nuclei. The time course of induction showed a close parallelism between the bound and free enzyme activities with the former always accounting for a higher percentage of the total activity as compared to the latter. Studies with cycloheximide indicated a half-life of around 3 hr for both the bound and free ALA synthetase. Actinomycin D and hemin prevented enzyme induction when administered along with DDC, but when administered 12 hr after DDC treatment Actinomycin D did not lead to a decay of either the bound or free enzyme activity and hemin inhibited the bound enzyme activity but not the free enzyme level. The molecular sizes of the mitochondrial and cytosolic ALA synthetase(s) were found to be similar on sephadex columns.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:42557
Deposited On:04 Jun 2011 11:23
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