Immunochemical detection of Z-DNA in rat pachytene spermatocytes

Satyanarayana, Karuturi ; Rao, Manchanahalli R. Satyanarayana (1989) Immunochemical detection of Z-DNA in rat pachytene spermatocytes Experimental Cell Research, 185 (2). pp. 319-326. ISSN 0014-4827

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001448...

Related URL: http://dx.doi.org/10.1016/0014-4827(89)90302-9

Abstract

Rat testicular nuclei have been probed for the presence of Z-DNA conformation by employing indirect immunofluorescence technique using anti-Z-DNA antibodies. Pachytene nuclei, in which meiotic recombination takes place, showed brighter fluorescence than the premeiotic and postmeiotic spermatogenic nuclei. Moreover, utilizing a novel chromatin immunoblotting tecnique, Z-DNA conformation was found to be enriched in the poly(ADP-ribosyl)ated chromatin domains of the pachytene nucleus.

Item Type:Article
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Deposited On:04 Jun 2011 10:19
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