FRET by FET and dynamics of polymer folding

Srinivas, Goundla ; Yethiraj, Arun ; Bagchi, Biman (2001) FRET by FET and dynamics of polymer folding Journal of Physical Chemistry B, 105 (13). pp. 2475-2478. ISSN 1089-5647

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Fluorescence resonance energy transfer (FRET) is a powerful tool for the investigation of the conformational and dynamic properties of macromolecules. In this work, we report Brownian dynamics simulations of FRET during polymer folding. The polymer molecule is modeled as a necklace of beads that interact via a site-site Lennard-Jones interaction, and FRET occurs via Forster energy transfer between the ends of the chain. The simulations demonstrate that FRET can act as a good marker of polymer folding only when the Forster radius (RF) is smaller than the root-mean-square radius (R0) of the polymer. FRET is sensitive to the early stages of polymer folding for RF ≤ R0 and to the late stages of polymer folding for RF « R0. This suggests that it might be necessary to employ more than one donor-acceptor pair in experiments aimed at probing the entire dynamics of polymer folding.

Item Type:Article
Source:Copyright of this article belongs to American Chemical Society.
ID Code:4222
Deposited On:13 Oct 2010 09:10
Last Modified:08 Jan 2011 11:19

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