Satellite DNA of Drosophila nasuta nasuta and D. n. albomicana: localization in polytene and metaphase chromosomes

Ranganath, H. A. ; Schmidt, E. R. ; Hägele, K. (1982) Satellite DNA of Drosophila nasuta nasuta and D. n. albomicana: localization in polytene and metaphase chromosomes Chromosoma, 85 (3). pp. 361-368. ISSN 0009-5915

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Official URL: http://www.springerlink.com/content/q9725j41211844...

Related URL: http://dx.doi.org/10.1007/BF00330358

Abstract

The DNA from the two Drosophila nasuta races, D. n. nasuta and D. n. albomicana was investigated by CsCl density gradient centrifugation. D. n. nasuta has one major AT-rich satellite DNA sequence with a density of 1.664g/cm3, while D. n. albomicana has at least three satellites with densities of 1.674g/cm3, 1.665g/cm3 and 1.661 g/cm3. The isolated satellite sequences hybridize in situ to all heterochromatic regions of all metaphase chromosomes of both races. In polytene chromosomes the satellite sequences hybridize exclusively to the chromocenter. All chromosomal regions hybridizing with the satellites show also bright quinacrine fluorescence.

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