Epidermal growth factor stimulates tyrosine phosphorylation of human glucocorticoid receptor in cultured cells

Rao, Kanury V. S. ; Fred Fox, C. (1987) Epidermal growth factor stimulates tyrosine phosphorylation of human glucocorticoid receptor in cultured cells Biochemical and Biophysical Research Communications, 144 (1). pp. 512-519. ISSN 0006-291X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...

Related URL: http://dx.doi.org/10.1016/S0006-291X(87)80539-9

Abstract

Human breast epithelial HBL100 cells, which bind both epidermal growth factor (EGF) and glucocorticoids, were labelled to steady state specific activity with 32Pi and the glucocorticoid receptor was immunoprecipitated from cell lysates with polyclonal antiserum GR884. Immuneprecipitated receptor was resolved by NaDodSO4-polyacrylamide gel electrophoresis and identified by autoradiography. Immunoprecipitated receptor also was characterized by western blot analysis and affinity labelling with [3H]dexamethasone-21-mesylate. Phosphoamino acid analysis of 32P-glucocorticoid receptor revealed 89% phosphoserine and 11% phosphotyrosine. Treatment of steady state 32Pi-labelled cells with EGF stimulated total and alkali-stable phosphorylation in the 97 kDa receptor band by about 35%. Prior incubation with dexamethasone inhibited EGF stimulated, alkali-stable phosphorylation of the 97 kDa glucocorticoid receptor band.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:41351
Deposited On:28 May 2011 08:05
Last Modified:28 May 2011 08:05

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