Rybak, S. M. ; Ramachandran, J. (1981) Primary culture of normal rat adrenocortical cells. II. Quantitation of steroid dehydrogenase stain In Vitro Cellular and Developmental Biology - Plant, 17 (7). pp. 605-611. ISSN 1054-5476
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Official URL: http://www.springerlink.com/content/867r7j573271j6...
Related URL: http://dx.doi.org/10.1007/BF02618459
Abstract
A simple, rapid procedure for the quantitation of the histochemical stain for Δ5-3β-dehydrogenase (3β-HSD) in cultured cells is described. Adrenocortical cells were stained for 3β-HSD and then solubilized with 0.5N NaOH. The absorbance of the solubilized cell solution was measured at 570 nm and a linear relationship was obtained between the number of cells and the intensity of the absorption. It is shown that the regulation of 3β-HSD activity in primary cultures of normal rat adrenocortical cells can be monitored by the quantitative histochemical assay. The results show a good correlation with the data obtained by a biochemical assay for the enzyme.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Society for In Vitro Biology. |
| Keywords: | Steroid Dehydrogenase; Staining Adrenocortical Cells; Quantitation |
| ID Code: | 40939 |
| Deposited On: | 25 May 2011 10:09 |
| Last Modified: | 25 May 2011 10:09 |
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