The synthesis and circular dichroism of a series of peptides possessing the structure (L-Tyrosyl-L-alanyl-L-glutamyl)n

Schechter, Bilha ; Schechter, Israel ; Ramachandran, Janakiraman ; Conway-jacobs, Abigail ; Sela, Michael (1971) The synthesis and circular dichroism of a series of peptides possessing the structure (L-Tyrosyl-L-alanyl-L-glutamyl)n European Journal of Biochemistry, 20 (3). pp. 301-308. ISSN 0014-2956

Full text not available from this repository.

Official URL:

Related URL:


Series of peptides possessing the structure (l-Tyr-l-Ala-l-Glu)n, with n=1,2,3,4,7,9, and 13 was synthesized. The oligomers up to n=4 were prepared by stepwise synthesis using the N-hydroxysuccinimide ester of the tripeptide derivative for the elongation of the chain. The oligomers with n=7,9, 13 were prepared by a polycondensation technique, followed by gel filtration. The circular dichroism spectra of the above oligopeptides were measured in the wavelength range 200-330 nm in a solution of 0.15 M sodium chloride −0.02 M sodium phosphate, pH 7.4, conditions at which the high molecular weight polytripeptide (Tyr-Ala-Glu)n exists in a helical conformation. The circular dichroic spectrum of the high molecular weight polymer exhibits two negative ellipticity bands: an intense band at 220 nm ([θ]max=8700) and a weak band at 273 nm ([θ]max=360). The tripeptide (n=1) exhibits a positive ellipticity band at 227 nm ([θ]max=4000) and a broad, positive, weak band at 270 nm ([θ]max=115). The other oligopeptides (n =2 to 13) exhibit positive bands in the 227 nm region, with ellipticity values that decrease as the degree of polymerization increases. Except for (Tyr-Ala-Glu)13, which has a negative peak centered at 216 nm, circular dichroic spectra of the other oligomers (n=1 to 9) in the 200-210 nm region resemble that of random coils. In the 260-290 nm region, the circular dichroic curves of the oligopeptides gradually approach the 272 nm band of the polypeptide (Tyr-Ala-Glu)n, as the degree of polymerization increases. Under physiological conditions, only the (Tyr-Ala-Glu)13 shows an indication of helical content.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
ID Code:40931
Deposited On:25 May 2011 10:03
Last Modified:25 May 2011 10:03

Repository Staff Only: item control page