Persistent activation of steroidogenesis in adrenocortical cells by photoaffinity labeling of corticotropin receptors

Ramachandran, J. ; Hagman, J. ; Muramoto, K. (1981) Persistent activation of steroidogenesis in adrenocortical cells by photoaffinity labeling of corticotropin receptors Journal of Biological Chemistry, 256 . pp. 11424-11427. ISSN 0021-9258

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Official URL: http://www.jbc.org/content/256/22/11424.short

Abstract

Photolysis of rat adrenocortical cells in the presence of the photoreactive derivative [(2-nitro-5-azidophenylsulfenyl)Trp9]-adrenocorticotropic hormone (2,5-NAPS-ACTH) at 24°C resulted in persistent activation of corticosterone production. The basal rate of steroidogenesis became maximal when photolysis was performed at 24°C but remained the same as that of control cells when irradiation was performed at 0°C. No increase in basal rate was observed with dark controls or cells photolyzed with [(2,4-dinitrophenylsulfenyl)Trp9]ACTH, a photoresistant analog of the hormone. Prephotolyzed 2,5-NAPS-ACTH failed to induce persistent activation. Both ACTH and 2,4-(dinitrophenylsulfenyl)Trp9-ACTH blocked the photo-induced activation of steroidogenesis elicited by 2,5-NAPS-ACTH. Under photolysis conditions which caused the basal rate of steroidogenesis to become maximal, a 3-fold increase in the basal rate of cAMP formation was observed.

Item Type:Article
Source:Copyright of this article belongs to The American Society for Biochemistry and Molecular Biology.
ID Code:40870
Deposited On:25 May 2011 09:22
Last Modified:17 May 2016 22:47

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