Van Dop, C. ; Tsubokawa, M. ; Bourne, H. R. ; Ramachandran, J. (1984) Amino acid sequence of retinal transducin at the site ADP-ribosylated by cholera toxin Journal of Biological Chemistry, 259 . pp. 696-698. ISSN 0021-9258
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Official URL: http://www.jbc.org/content/259/2/696.short
Abstract
Transducin was [32P]ADP-ribosylated by cholera toxin in bovine retinal rod outer segments and then partially purified on ω-amino octyl agarose to remove other ADP-ribosylated proteins. Trypsin digestion of the ADP-ribosylated transducin and further purification using boronate-polyacrylamide beads and high performance liquid chromatography yielded a single radiolabeled tetrapeptide, Ser-Arg-Val-Lys. The ADP-ribose is linked to the guanidinium group of arginine.
| Item Type: | Article |
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| Source: | Copyright of this article belongs to The American Society for Biochemistry and Molecular Biology. |
| ID Code: | 40864 |
| Deposited On: | 25 May 2011 09:21 |
| Last Modified: | 17 May 2016 22:47 |
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