Human leukocyte interferon has no structural or biological relationship to corticotropin

Wetzel, Ronald ; Levine, Howard L. ; Hagman, J. ; Ramachandran, J. (1982) Human leukocyte interferon has no structural or biological relationship to corticotropin Biochemical and Biophysical Research Communications, 104 (3). pp. 944-949. ISSN 0006-291X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000629...

Related URL: http://dx.doi.org/10.1016/0006-291X(82)91340-7

Abstract

In view of recent reports that certain preparations of human leukocyte interferons are structurally and biologically related to the pituitary hormones corticotropin (ACTH) and β-endorphin, we have investigated the properties of two human leukocyte interferons (IFN-α) prepared by recombinant DNA technology. The antiviral activities of purified IFN-αA and IFN-αD were not affected by a large molar excess of ACTH antiserum nor did ACTH interfere in interferon immunoassays. Neither IFN-αA, IFN-αD nor pepsin digests of these proteins were able to stimulate steroidogenesis in adrenocortical cells. There was no cross reaction between ACTH antiserum and the two leukocyte interferons or the pepsin digests of the interferons. These results cast doubt on recent proposals that some of interferon's biological effects are mediated by ACTH or β-endorphin-related fragments of the interferon molecule.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:40835
Deposited On:25 May 2011 07:14
Last Modified:25 May 2011 07:14

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