Responses of Y1 adrenocortical tumor cells to o-nitrophenyl sulfenyl ACTH

Rae, Patricia A. ; Zinman, Howard ; Ramachandran, J. ; Schimmer, Bernard P. (1980) Responses of Y1 adrenocortical tumor cells to o-nitrophenyl sulfenyl ACTH Molecular and Cellular Endocrinology, 17 (3). pp. 171-179. ISSN 0303-7207

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NPS(o-nitrophenyl sulfenyl)-ACTH stimulated steroidogenesis in Y1 cells to the same maximum level as did ACTH, but with a 60-fold lower potency than the native hormone. NPS-ACTH also stimulated the extracellular accumulation of cAMP in Y1 cells with lower potency than the unmodified hormone. The amount of cAMP accumulated in the presence of NPS-ACTH approached 75% of the maximum achieved with ACTH. In Y1 plasma membranes, NPS-ACTH was a partial agonist of adenylate cyclase activity, with an efficacy dependent upon the type of guanyl nucleotide present. The steroidogenic responses of two Y1(Kin) mutants and two Y1(Cyc) mutants to NPS-ACTH paralleled their responses to ACTH and reflected closely their defects in cAMP-dependent protein kinase and ACTH-sensitive adenylate cyclase activity. These data imply an obligatory role for adenylate cyclase and cAMP-dependent protein kinase activities in stimulation of steroidogenesis in Y1 cells by NPS-ACTH cyclic.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:AMP; Steroidogenesis; cAMP-dependent Protein Kinase; Y1 Mutants
ID Code:40728
Deposited On:25 May 2011 05:25
Last Modified:25 May 2011 05:25

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