Ramachandran, J. (1971) A new simple method for separation of adenosine 3',5'-cyclic monophosphate from other nucleotides and its use in the assay of adenyl cyclase Analytical Biochemistry, 43 (1). pp. 227-239. ISSN 0003-2697
Full text not available from this repository.
Official URL: http://linkinghub.elsevier.com/retrieve/pii/000326...
Related URL: http://dx.doi.org/10.1016/0003-2697(71)90128-X
Abstract
A rapid, simple, one-step procedure is described for the separation of cyclic AMP from ATP, ADP, AMP, and P1 based on selective adsorption of all nucleotides except cyclic AMP on hydrous aluminum oxide. This separation procedure has been incorporated into the assay for adenyl cyclase using ATP-α -32P as substrate. The new method was employed to study the stimulation of rat adrenal adenyl cyclase by ACTH and fluoride. The purity of the cyclic AMP isolated from hydrous alumina columns was established by chromatography on Dowex 50 H+ and Dowex 1 Cl− ion-exchange resins thin-layer chromatography on silica gel in two solvents, paper electrophoresis, Ba-Zn precipitation, and crystallization to constant specific radioactivity.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 40727 |
| Deposited On: | 25 May 2011 05:20 |
| Last Modified: | 25 May 2011 05:20 |
Repository Staff Only: item control page
