NADPH cytochrome P-450 reductase in rat, mouse and human brain

Ravindranath, Vijayalakshmi ; Anandatheerthavarada, Hindupur K. ; Shankar, Susarla K. (1990) NADPH cytochrome P-450 reductase in rat, mouse and human brain Biochemical Pharmacology, 39 (6). pp. 1013-1018. ISSN 0006-2952

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NADPH cytochrome P-450 reductase (P-450 reductase), an essential component of the cytochrome P-450 mono-oxygenase system, has been estimated in rat and mouse brain, and seven human brains obtained at autopsy. The ratio of cytochrome P-450 to P-450 reductase is lower in the rat and mouse brains (2.5-4.0) as compared to the respective livers (10.0-11.0). The rat and mouse brain P-450 reductase were immunologically similar to the rat liver P-450 reductase as examined by immunochemical inhibition, Ouchterlony double diffusion and immunoblot. The antisera to rat liver P-450 reductase inhibited rat brain aminopyrine N-demethylase activity to the same extent as NADPH cytochrome c reductase, suggesting that the level of P-450 reductase controls the rate of this cytochrome P-450 mediated activity. The human brain NADPH cytochrome c reductase exhibited regional variation, maximal activity being observed in the brain stem region. Immunochemical inhibition and immunoblot studies revealed immunological cross-reactivity between rat liver reductase and human brain medulla, while none was observed in cortex or cerebellum. Immunocytochernical studies on human brain medulla using antisera to rat liver P-450 reductase indicated localization of the P-450 reductase in neuronal cell body.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:40495
Deposited On:24 May 2011 13:28
Last Modified:24 May 2011 13:28

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