Isolation of intact mesophyll cells from the leaves of higher plants

Rajendrudu, G. ; Madhusudana Rao, I. ; Raghavendra, A. S. ; Rama Das, V. S. (1979) Isolation of intact mesophyll cells from the leaves of higher plants Proceedings of the Indian Academy of Sciences, Section B, 88 (2). pp. 143-154. ISSN 0370-0097

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A total of 146 species of angiosperms belonging to 35 taxonomically diverse families were screened for the isolation of living mesophyll cells from the leaves. Seventy-three species belonging to 22 families, on mild maceration in mortar with the isolation medium (pH 5.8) containing 0.7 M mannitoJ, 2 mM EDTA, 5 mM MgCl2, 5 mM K2HPO4 and 1 mM NaNO3, followed by fractional centrifugation, yielded intact mesophyll cells as seen under a research microscope. The high frequency of cell release, associated with the high percentage recovery of chlorophyll in cells was a common feature of most of the plant species examined by us. Nearly 87% of the chlorophyll present in the leaf could be recovered from the isolated cells in Dolichos lablab. The isolated cells retained active photosynthetic carbon metabolism as evidenced by high rates of ferricyanide reduction as well as carbon assimilation.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Isolated Cells; Photosynthesis; Maceration
ID Code:40254
Deposited On:23 May 2011 09:05
Last Modified:17 May 2016 22:24

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