Beneficial interaction between photosynthesis and respiration in mesophyll protoplasts of pea during short light-dark cycles

Abstract

The respiratory uptake or photosynthetic evolution of oxygen by mesophyll protoplasts of pea (Pisum sativum L. cv. Arkel) were monitored during successive short. (3-5 min) cycles of darkness and illumination. The rate of respiration was nearly doubled after 3-4 short periods of illumination while there was a 15-20% enhancement in photosynthesis with cycles of illumination and darkness preceding illumination. Such interaction between photosynthesis and respiration was statistically significant when bicarbonate was present in the reaction medium. The inhibitors of photosynthesis [3(3,4-dichlorophenyl)-l,l-dimethylurea (DCMU), glyceraldehyde] decreased respiration after periods of illumination, whereas inhibitors of respiratory electron transport (Rotenone, antimycin A, NaN₃) suppressed photosynthesis, as well. We suggest that a rapid beneficial interaction exists between photosynthesis and respiration in protoplasts, even during short cycles of light and darkness.