Malonate-inhibition of allosteric phosphoenolpyruvate carboxylase from Setaria italica

Raghavendra, A. S. ; Das, V. S. R. (1975) Malonate-inhibition of allosteric phosphoenolpyruvate carboxylase from Setaria italica Biochemical and Biophysical Research Communications, 66 (1). pp. 160-165. ISSN 0006-291X

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00062...

Related URL: http://dx.doi.org/10.1016/S0006-291X(75)80308-1

Abstract

Malonate inhibited phosphoenolpyruvate carboxylase activity in crude extracts of Setaria italica Beauv. and Pennisetum typhoides S & H leaves. Kinetic studies revealed the competetive inhibition of partially purified phosphoenolpyruvate carboxylase preparation from Setaria italica. Glucose-6-phosphate activated the enzyme. Oxalacetate also competetively inhibited the enzyme and malate in a noncompetetive manner. Results clearly indicated the allosteric nature of phosphoenolpyruvate carboxylase from Setaria italica.

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