Effect of gamma-radiation on the structure and function of yeast membrane

khare, Shailja ; Trivedi, Akhilesh ; Kesavan, P. C. ; Prasad, R. (1982) Effect of gamma-radiation on the structure and function of yeast membrane International Journal of Radiation Biology, 42 (4). pp. 369-383. ISSN 0955-3002

Full text not available from this repository.

Official URL: http://informahealthcare.com/doi/abs/10.1080/09553...

Related URL: http://dx.doi.org/10.1080/09553008214551301


A decrease in the influx of several amino acids was observed following gamma-irradiation. At low dose (2.5 Gy), which does not affect cell survival, a stimulation in the uptake was visible; moreover, sulphydryl loss and lipid peroxidation were also evident. With further increase in the dose of radiation, a parallel increment in the loss of sulphydryl groups and production of malonaldehyde was observed. Radioprotectors like L-cysteine and dithiothreitol were shown to shield the radiation-induced loss of sulphydryl and damage to transport and survival. Reduced glutathione, on the other hand, exhibited protection at the level of sulphydryl damage only. N-ethylmaleimide, a well known hypoxic cell radiosensitizer, enhanced the radiosensitivity with respect to survival; it, however, had no effect on amino acid transport. Oxygen enhancement of radiation damage to transport and cell survival and the radioprotection by sodium formate under these circumstances, and more so by anoxia, were demonstrated. The results indicate that the manifestation of damage to membrane structure and function precedes any observable loss of survival.

Item Type:Article
Source:Copyright of this article belongs to Informa plc.
ID Code:39398
Deposited On:12 May 2011 08:13
Last Modified:12 May 2011 08:13

Repository Staff Only: item control page