CDR1, a multidrug resistance gene from Candida albicans, contains multiple regulatory domains in its promoter and the distal AP-1 element mediates its induction by miconazole

Puri, Neeti ; Krishnamurthy, S. ; Habib, Saman ; Hasnain, Seyed E. ; Goswami, Shyamal K. ; Prasad, Rajendra (1999) CDR1, a multidrug resistance gene from Candida albicans, contains multiple regulatory domains in its promoter and the distal AP-1 element mediates its induction by miconazole FEMS Microbiology Letters, 180 (2). pp. 213-219. ISSN 0378-1097

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1574-...

Related URL: http://dx.doi.org/10.1111/j.1574-6968.1999.tb08798.x

Abstract

We previously demonstrated that the CDR1 gene, encoding a multidrug transporter in Candida albicans, is differentially upregulated by various drugs and steroids. In order to get an insight into the molecular basis of the induction of this gene we analyzed its promoter region. The transcription start site was mapped to 63 nucleotides upstream of the initiating ATG. Reporter assays revealed the presence of four upstream activating and four upstream repressing sequence domains along the entire promoter. Like the native gene, promoter-luciferase recombinants showed enhanced activity in response to various stresses like drugs, human steroid hormones and heavy metals. Mutational analysis demonstrated that while the proximal promoter (−345/+1) contains all the regulatory domains required for its induction by various other stresses, the miconazole response is mediated via the distal promoter (−857/−1147), harboring an AP-1 site. The involvement of the AP-1 element in mediating the latter effect was evident by an increase in AP-1 binding activity following miconazole treatment.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:CDR1; ABC Transporter; Gene Expression; Promoter; AP-1; Candida albicans
ID Code:39368
Deposited On:12 May 2011 06:31
Last Modified:17 May 2016 21:51

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