A retinal ganglion cell neurotrophic factor purified from the superior colliculus

Schulz, Mark ; Raju, Trichur ; Ralston, Gregory ; Bennett, Max R. (1990) A retinal ganglion cell neurotrophic factor purified from the superior colliculus Journal of Neurochemistry, 55 (3). pp. 832-841. ISSN 0022-3042

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1471-...

Related URL: http://dx.doi.org/10.1111/j.1471-4159.1990.tb04567.x


Dissociated neonatal rat retinal ganglion cells can be maintained by the addition of an extract from the neonatal superior colliculus. This extract can support 95% of ganglion cells over 24 h in culture; in addition it promotes the expression of neurites from these cells. This report describes the purification of a neurotrophic factor from the superior colliculus which supports the survival of 80% of retinal ganglion cells over 24 h in vitro. The purification procedure involves a combination of dye-ligand, anion-exchange, and molecular sieve chromatography. The purified neurotrophic factor has a Stokes radius of approximately 200 Å using molecular sieve chromatography in the presence of a chaotropic agent. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified factor indicates that it is a glycoprotein that migrates with a molecular mass >400 kDa. Further characterization of this high-molecular-mass glycoprotein by enzymatic digestion demonstrated that it is a chondroitin sulfate pro-teoglycan. This factor is clearly distinguishable from other neurotrophic factors that have an effect on retinal ganglion cells such as brain-derived neurotrophic factor and fibroblast growth factor. The chondroitin sulfate proteoglycan from the neonatal superior colliculus is the first proteoglycan to be identified as a neurotrophic factor.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Retina; Ganglion Cell; Superior Colliculus; Neurotrophic Factor; Proteoglycan
ID Code:38527
Deposited On:30 Apr 2011 08:48
Last Modified:30 Apr 2011 08:48

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