Expression of galactoside-specific endogenous lectins and their ligands in human oral squamous cell carcinoma

Kannan, S. ; Gabius, Hans-Joachim ; Jagadeesh Chandran, G. ; Pillai, M. Radhakrishna ; Nalinakumari, K. R. ; Krishnan Nair, M. (1994) Expression of galactoside-specific endogenous lectins and their ligands in human oral squamous cell carcinoma Cancer Letters, 85 (1). pp. 1-7. ISSN 0304-3835

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/030438...

Related URL: http://dx.doi.org/10.1016/0304-3835(94)90231-3

Abstract

Human endogenous lectins have a wide spectrum of biological functions. The present study analyses the expression of β-galactoside specific and N-acetyl-D-galactosamino specific endogenous lectins in oral squamous cell carcinomas using biotinylated neoglycoproteins. The expression pattern of β-galactosyl-containing glycoconjugates or ligands of β-galactoside specific lectins in these tissues was also studied using an endogenous biotinylated lectin, the human 14-kDa lectin. For comparison a galactoside specific plant lectin from mistletoe, Viscum album was also employed. The results demonstrate that oral squamous cell carcinomas mainly express accessible binding sites for lactosylated neoglycoprotein (90%) while few carcinomas expressed mild amount of N-acetyl-D-galactosamine specific binding sites (40%). There was no difference in the binding patterns of these probes between well and less differentiated carcinomas. Expression of these neoglycoprotein binding sites were mostly concentrated in immature basaloid cells, indicating a possible association with cell proliferation. The binding pattern of D-galactosyl specific lectins (human 14-kDa and mistletoe lectins) showed conspicuous differences. This feature emphasizes the caution that needs to be exercised in interpreting the biological significance of results attained using plant lectins on human tissue.

Item Type:Article
Source:Copyright of this articles belongs to Elsevier Science.
Keywords:Endogenous Lectins; Glycohistochemistry; Neoglycoproteins; Oral Carcinoma
ID Code:38306
Deposited On:03 May 2011 13:11
Last Modified:03 May 2011 13:11

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