Involvement of G-proteins, calmodulin and tagetitoxin-sensitive RNA polymerase in light-regulated expression of plastid genes (psbA, psaA and rbcL) in rice (Oryza sativa L.)

Dhingra, Amit ; Khurana, Jitendra P. ; Tyagi, Akhilesh K. (2004) Involvement of G-proteins, calmodulin and tagetitoxin-sensitive RNA polymerase in light-regulated expression of plastid genes (psbA, psaA and rbcL) in rice (Oryza sativa L.) Plant Science, 166 (1). pp. 163-168. ISSN 0168-9452

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S01689...

Related URL: http://dx.doi.org/10.1016/j.plantsci.2003.09.003

Abstract

The regulation of chloroplast gene expression by light involves multiple signaling components. In an earlier study, we demonstrated the role of calcium and phosphorylation in regulating the expression of photosynthesis-related plastid genes, psbA, psaA and rbcL, using rice as a model monocot system. This work has been extended further to examine the possible involvement of heterotrimeric GTP-binding proteins and calmodulin. Vacuum infiltration of 5-day-old etiolated rice seedlings with G-protein agonists, cholera toxin and GTPγS, increased the steady-state transcript levels of the plastid genes. The antagonists/inhibitors of calmodulin action, trifluoperazine and W7, inhibited the light-induced increase in steady-state transcript levels of these genes. The light-regulated expression of photosynthetic genes was also adversely affected by tagetitoxin, a specific inhibitor of plastid-encoded RNA polymerase. These results indicate the involvement of various signaling components in transduction of light signal that probably also recruits PEP to regulate plastid gene expression.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Calmodulin; Gene Expression; G-Proteins; Light; Oryza sativa L.; Plastid-encoded RNA Polymerase (PEP)
ID Code:38216
Deposited On:25 Apr 2011 13:13
Last Modified:17 May 2016 21:08

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