Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice

Jain, Mukesh ; Khurana, Jitendra P. (2009) Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice FEBS Journal, 276 (11). pp. 3148-3162. ISSN 1742-464X

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1742-...

Related URL: http://dx.doi.org/10.1111/j.1742-4658.2009.07033.x

Abstract

Auxin influences growth and development in plants by altering gene expression. Many auxin-responsive genes have been characterized in Arabidopsis in detail, but not in crop plants. Earlier, we reported the identification and characterization of the members of the GH3, Aux/IAA and SAUR gene families in rice. In this study, whole genome microarray analysis of auxin-responsive genes in rice was performed, with the aim of gaining some insight into the mechanism of auxin action. A comparison of expression profiles of untreated and auxin-treated rice seedlings identified 315 probe sets representing 298 (225 upregulated and 73 downregulated) unique genes as auxin-responsive. Functional categorization revealed that genes involved in various biological processes, including metabolism, transcription, signal transduction, and transport, are regulated by auxin. The expression profiles of auxin-responsive genes identified in this study and those of the members of the GH3, Aux/IAA, SAUR and ARF gene families were analyzed during various stages of vegetative and reproductive (panicle and seed) development by employing microarray analysis. Many of these genes are, indeed, expressed in a tissue-specific or developmental stage-specific manner, and the expression profiles of some of the representative genes were confirmed by real-time PCR. The differential expression of auxin-responsive genes during various stages of panicle and seed development implies their involvement in diverse developmental processes. Moreover, several auxin-responsive genes were differentially expressed under various abiotic stress conditions, indicating crosstalk between auxin and abiotic stress signaling.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Abiotic Stress; Auxin; Microarray Analysis; Reproductive Development; Rice (Oryza sativa)
ID Code:38192
Deposited On:28 Apr 2011 08:34
Last Modified:17 May 2016 21:06

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