RAPD and RFLP mapping of the bacterial blight resistance gene xa-13 in rice

Zhang, G. ; Angeles, E. R. ; Abenes, M. L. P. ; Khush, G. S. ; Huang, N. (1996) RAPD and RFLP mapping of the bacterial blight resistance gene xa-13 in rice Theoretical and Applied Genetics (TAG), 93 (1-2). pp. 65-70. ISSN 0040-5752

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Official URL: http://www.springerlink.com/content/u5u3t845866602...

Related URL: http://dx.doi.org/10.1007/BF00225728

Abstract

Bacterial blight (BB) caused by Xanthomonas oryzae pv oryzae (Xoo) is one of the most serious diseases of rice. The recessive gene xa-13 confers resistance to Philippine race 6 of Xoo. To tag xa-13 with molecular markers, RAPD analysis was conducted with the combined use of near-isogenic lines and bulked segregant analysis. From the survey of 260 arbitrary 10-nucleotide primers, one primer (OPAC05) was detected to amplify specifically a 0.9-kb band from the DNA of susceptible plants. The distance between the RAPD marker OPAC05-900 and xa-13 was estimated to be 5.3 cM. The RAPD marker was then mapped on chromosome 8 using a mapping population of doubled haploid lines derived from the cross of IR64/Azucena. The linkage between RFLP markers and the RAPD marker was analyzed using an F2 population of 135 plants derived from a cross between a near-isogenic line for xa-13, IR66699-5-5-4-2, and IR24. No recombinants were found between RZ28 and CDO116 and their distance from xa-13 was estimated to be 4.8 cM. RG136 was located at 3.7 cM on the other side of xa-13. The mapping of xa-13 with closely linked DNA markers provides the basis for marker-aided selection for rice improvement.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Molecular Mapping; Xanthomonas oryzae; Resistance Gene; Marker-aided Selection; Oryza sativa
ID Code:37583
Deposited On:23 Apr 2011 13:00
Last Modified:17 May 2016 20:29

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