A simple procedure for the manual isolation and identification of plant heterokaryons

Patnaik, G. ; Cocking, E. C. ; Hamill, J. ; Pental, D. (1982) A simple procedure for the manual isolation and identification of plant heterokaryons Plant Science Letters, 24 (1). pp. 105-110. ISSN 0304-4211

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/030442...

Related URL: http://dx.doi.org/10.1016/0304-4211(82)90014-1

Abstract

A simple procedure is described for the aseptic manual isolation of individual heterokaryons. Heterokaryons were identified with bright field illumination using an inverted microscope and isolated by means of a micro-manipulator and capillary pipette coupled to a specially constructed syringe. When cell suspension protoplasts were labelled with fluorescein isothiocyanate and fused with mesophyll protoplasts, the heterokaryons exhibited an apple green cytoplasmic fluorescence (from cell suspension protoplasts) and a red chloroplast fluorescence (from mesophyll protoplasts). By this double fluorescence procedure, these manually isolated heterokaryons, identified initially using brightfield could be confirmed as heterokaryons.

Item Type:Article
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ID Code:36667
Deposited On:06 Jul 2011 10:07
Last Modified:06 Jul 2011 10:07

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