A sensitive sandwich ELISA for buffalo prolactin

Panchal, Manoj ; Muralidhar, K. ; Gupta, S. K. (2007) A sensitive sandwich ELISA for buffalo prolactin Journal of Immunoassay and Immunochemistry, 28 (2). pp. 147-154. ISSN 1532-1819

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Official URL: http://www.informaworld.com/index/773637819.pdf

Related URL: http://dx.doi.org/10.1080/15321810701212062


Two murine monoclonal antibodies have been developed against the buffalo prolactin (buPRL). These were designated as 1501 and 1504. Using two MAbs and anti-buPRL rabbit serum, an analysis was performed for the development of a sandwich ELISA (sELISA). The 1504/buPRL/anti-buPRL rabbit serum system was found feasible for sELISA. The sELISA had a sensitivity of 156 pg/mL. The buffalo serum sample showed a parallelism with the standard curve. The intra- and inter-assay coefficients of variance were 8.4% and 9.06%, respectively. These data proved the validity of the assay.

Item Type:Article
Source:Copyright of this article belongs to Taylor and Francis Group.
Keywords:buPRL; Sandwich ELISA; Monoclonal Antibodies
ID Code:36627
Deposited On:14 Apr 2011 14:14
Last Modified:21 Apr 2011 13:06

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