Panchal, Manoj ; Muralidhar, K. ; Gupta, S. K. (2007) A sensitive sandwich ELISA for buffalo prolactin Journal of Immunoassay and Immunochemistry, 28 (2). pp. 147-154. ISSN 1532-1819
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Official URL: http://www.informaworld.com/index/773637819.pdf
Related URL: http://dx.doi.org/10.1080/15321810701212062
Abstract
Two murine monoclonal antibodies have been developed against the buffalo prolactin (buPRL). These were designated as 1501 and 1504. Using two MAbs and anti-buPRL rabbit serum, an analysis was performed for the development of a sandwich ELISA (sELISA). The 1504/buPRL/anti-buPRL rabbit serum system was found feasible for sELISA. The sELISA had a sensitivity of 156 pg/mL. The buffalo serum sample showed a parallelism with the standard curve. The intra- and inter-assay coefficients of variance were 8.4% and 9.06%, respectively. These data proved the validity of the assay.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Taylor and Francis Group. |
| Keywords: | buPRL; Sandwich ELISA; Monoclonal Antibodies |
| ID Code: | 36627 |
| Deposited On: | 14 Apr 2011 14:14 |
| Last Modified: | 21 Apr 2011 13:06 |
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