Are sheep and buffalo prolactins sulfated?

Kohli, Rita ; Chadha, Neeraja ; Muralidhar, K. (1987) Are sheep and buffalo prolactins sulfated? Biochemical and Biophysical Research Communications, 149 (2). pp. 515-522. ISSN 0006-291X

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Radioactive sulfate (35SO42-) has been shown to be incorporated into immunoprecipitable prolactin-like material from incubated minces of sheep and buffalo pituitaries. The 35S-labelled prolactin could be purified by standard procedures. On SDS-PAGE, the 35S-labelled prolactin rich fraction gives two major Coomassie blue bands around 25KDa and these on Western blot analysis gave positive bands. Radioactive [14C]-mannose was also found incorporated into the prolactin like material. The nature of sulphate link to the peptide is not known. It could be sugar-SO4 and/or Tyrosine-SO4.

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