Chromosomal locations of ten isozyme loci in rice (Oryza sativa L.) through trisomic analysis

Abstract

Chromosomal locations of 10 isozyme loci in rice (Oryza sativa L.) were determined through trisomic analysis. All 10 genes produced altered allozyme banding patterns in specific F₁ trisomics. This served as the primary source of evidence for chromosome locations of Est-5, Icd-1, Acp-1, and Pgd-1. The locations of Amp-1, Amp-2, Amp-4, Pox-5, Got-1, and Cat-1 were further confirmed from segregation data in BC₁ generations, as the ratios deviated significantly from 1:1 in the critical trisomics but agreed with the expected trisomic ratios. Triallelic heterozygotes were recovered for Amp-1 and Amp-2. On the basis of these data Got-1, Est-5, and Icd-1 were located to chromosome 1, Amp-1 to chromosome 2, Cat-1 and Pox-5 to chromosome 3, Acp-1 to chromosome 6, Amp-2 and Amp-4 to chromosome 8, and Pgd-1 to chromosome 11. Because Acp-2 and Pox-2 are known to be linked with Acp-1, they must also be on chromosome 6. The gene order and recombination values between isozyme loci on chromosomes 3, 6, 8, and 11 are presented.