Presence of tyrosine-O-sulfate in sheep pituitary prolactin

Kohli, Rita ; Chadha, Neeraja ; Muralidhar, K. (1988) Presence of tyrosine-O-sulfate in sheep pituitary prolactin FEBS Letters, 242 (1). pp. 139-143. ISSN 0014-5793

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When the metabolically obtained 35S-labelled sheep pituitary prolactin-rich fraction was subjected to chemical deglycosylation the radioactivity was retained in the immunoprecipitable prolactin. 35S-labelled prolactin-rich pituitary extract was fractionated on SDS-PAGE and protein was extracted from prolactin positive bands. When the extracted 35S-labelled prolactin was hydrolysed by alkali and then chromatographed on a thin layer of silica, it showed the presence of a radioactive compound which had an Rf value identical to the standard Tyr-O-SO4 synthesized and characterized in our laboratory.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Prolactin; Tyrosine-O-SO4; Immunoprecipitation; (Sheep Pituitary)
ID Code:36310
Deposited On:14 Apr 2011 14:12
Last Modified:17 May 2016 19:16

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