Genetic analysis of total glucosinolate in crosses involving a high glucosinolate Indian variety and a low glucosinolate line of Brassica juncea

Abstract

Analysis of the glucosinolate content and composition by high-pressure liquid chromatography indicated that varieties of Brassica juncea bred and grown in India have a high glucosinolate content characterized by the presence of 2-propenyl (allyl) and 3-butenyl as the major and 4-pentenyl as the minor fractions. In contrast, the B. juncea germplasm from other countries is characterized by the presence of 2-propenyl as the major glucosinolate fraction, trace amounts of 3-butenyl and a total lack of the 4-pentenyl types. In order to transfer the low glucosinolate trait to Indian B. juncea, the inheritance of total glucosinolates was investigated using doubled haploid (DH) populations derived from F1 (DH₁) and BC₁ (BC₁DH) of a cross between 'Varuna' (the most widely cultivated high glucosinolate variety of India) and 'Heera' (a non-allyl type low glucosinolate line). A total of 752 DH₁ and 1263 BC₁DH gave rise to seven and 11 low glucosinolate (containing less than 18 μmol/g seed) individuals, respectively. On the basis of the frequency of the low glucosinolate individuals, the total glucosinolate was found to be under the control of seven genes. There was presence of both allyl and non-allyl types in DH₁ and BC₁DH low-glucosinolate individuals and absence of 3-butenyl glucosinolate in some of the BC₁DH low glucosinolate individuals, indicating segregation for these fractions in the population. The size of the segregating DH population proved to be crucial for precise determination of the number of genes controlling the trait. Because of the large number of genes involved, incorporation of low glucosinolate trait in Indian B. juncea should be approached through doubled haploid (DH) breeding.