Synthesis of a substance exhibiting citrovorum factor activity by Lactobacillus arabinosus 17-5

Mitbander, V. B. ; Sreenivasan, A. (1954) Synthesis of a substance exhibiting citrovorum factor activity by Lactobacillus arabinosus 17-5 Archives of Microbiology , 21 (1). pp. 69-79. ISSN 0302-8933

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Official URL: http://www.springerlink.com/content/nu50481wk74162...

Related URL: http://dx.doi.org/10.1007/BF00412606

Abstract

Growing and respiring cells of Lactobacillus arabinosus elaborate citrovorum factor activity from p-aminobenzoic acid. Pteroylglutamic acid is a step in this conversion. That the activity synthesized is apparently different from the citrovorum factor is inferable from observations on inhibition analysis, differential distribution between immiscible solvents at various pH and bioautographic studies. Ascorbic acid aids in the synthesis of citrovorum factor active substance. Formate and "formyl" precursors have stimulatory effect on this synthesis but only in presence of ascorbic acid.

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