Biosynthesis of choline in vitro by rat liver

Dalal, F. R. ; Noronha, J. M. ; Sreenivasan, A. (1961) Biosynthesis of choline in vitro by rat liver Nature, 190 (4772). pp. 267-268. ISSN 0028-0836

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In conformity with the observation that only one of the three methyl groups of choline, after oxidation to betaine, can be transferred to homocysteine, it was shown from in vivo studies that only the conversion of dimethylethanolamine to choline is a trans-methylation, the influence of folic acid being confined to steps leading to the formation of monomethylethanolamine and dimethylethanolamine from ethanolamine. The present in vitro experiments were carried out to ascertain, more directly, the origin of the methyl groups of choline, and their relationship to folic acid.

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