Vitamin B12 binding by rat serum protein fractions

Mulgaonkar, Anand G. ; Sreenivasan, A. (1959) Vitamin B12 binding by rat serum protein fractions Archives of Biochemistry and Biophysics, 80 (1). pp. 22-25. ISSN 0003-9861

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/000398...

Related URL: http://dx.doi.org/10.1016/0003-9861(59)90336-4

Abstract

Vitamin B12 in normal rat serum is found to exist largely in the bound form and almost entirely associated with α2-globulin. With increasing in vitro additions of the vitamin to serum, up to 1.5 mμg./ml., the vitamin is chiefly recovered in the α2- and β-globulin fractions, the bound vitamin being confined to the α2-fraction. Any extra vitamin B12 added migrates in the free state with the γ-globulin fraction.

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