Role of cation in the dephosphorylation of phosphoproteins by alkali

Sundararajan, T. A. ; Sampath Kumar, K. S. V. ; Sarma, P. S. (1958) Role of cation in the dephosphorylation of phosphoproteins by alkali Biochimica et Biophysica Acta, 28 . pp. 148-158. ISSN 0006-3002

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1. 1. It has been observed that alkalis differ widely in their ability to dephosphorylate phosphoproteins. The hydroxides of calcium, strontium and barium were thus much more effective as dephosphorylating agents, compared with those of sodium and potassium. Ammonia, which was ineffective by itself, readily dephosphorylated phosvitin in the presence of certain metal ions. It is concluded from these studies that the dephsphorylation of phosphoproteins is catalysed by metal ion and that the dephosphorylating action of an alkali on phosphoprotein is influenced to a large extent by the nature of the cation present in it. 2. 2. Dephosphorylation studies carried out on different phosphoprotein substrates indicate the presence in these of phosphate groupings resistant to dephosphorylation. Several explanations have been offered to account for this observation. 3. 3. Phosphoprotein fraction obtained from tissues by Schneider's procedure has been shown to resemble other phosphoproteins in its dephosphorylation behaviour towards alkali and enzyme. 4. 4. An improved procedure for the preparation of alkali-dephosphorylated casein, which involves the use of dilute barium hydroxide as dephosphorylating agent, and a chemical method for the preparation of a phosphopeptone from casein, have been described.

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