The utilization of carbon-1 compounds by plants: II. The formation and metabolism of formate by higher plant tissues

Cossins, E. A. ; Sinha, S. K. (1965) The utilization of carbon-1 compounds by plants: II. The formation and metabolism of formate by higher plant tissues Biochemistry and Cell Biology, 43 (6). pp. 685-698. ISSN 0829-8211

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Related URL: http://dx.doi.org/10.1139/o65-079

Abstract

Tissue slices of carrot root and sunflower cotyledons readily produce formate-C14 when incubated with glyoxylate-1,2-C14 and glycine-2-C14 solutions. Formate-C14 feeding experiments using a wide variety of higher plant tissues have shown that this compound is readily oxidized to carbon dioxide. This oxidation probably involves a formic dehydrogenase system which has been demonstrated to occur in several of the tissues. Large amounts of formate-C14 were also incorporated into serine, methionine, and methionine sulfoxide. Degradations of the serine produced from formate-C14 showed that the bulk of the C14 was present in the 3 position. The incorporation of formate into serine by radish cotyledons was stimulated under anaerobic conditions and by additions of glycine. Synthesis of methionine and methionine sulfoxide from formate-C14 was stimulated by additions of homocysteine. The results are interpreted as an indication that formate-C14 can be oxidized to carbon dioxide by formic dehydrogenase and can serve as a precursor of carbon-1 units that are to be utilized for the biosynthesis of serine and methionine.

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ID Code:35256
Deposited On:11 Apr 2011 12:45
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