Oxidation of 2-mercaptoethanol in the presence of tris buffer

Koundal, Kirpa R. ; Sawhney, Surinder K. ; Sinha, Suresh K. (1983) Oxidation of 2-mercaptoethanol in the presence of tris buffer Phytochemistry, 22 (10). pp. 2183-2184. ISSN 0031-9422

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00319...

Related URL: http://dx.doi.org/10.1016/S0031-9422(00)80142-9


2-Mercaptoethanol is oxidized by molecular oxygen, especially at high pH. This reaction is markedly increased by primary amines such as asparagine, glutamine and their corresponding amino acids and by the buffer tris(hydroxymethyl)aminomethane. Thus, the use of 2-mercaptoethanol in Tris buffer may present several complications: an uptake of oxygen which could be confused with metabolic activity; destruction of the protective mercaptoethanol; and by inference, quite possibly an enhanced oxidation of the protein sulphydryls in need of protection.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:2-Mercaptoethanol; Oxidation; Tris Buffer
ID Code:35239
Deposited On:11 Apr 2011 12:59
Last Modified:11 Apr 2011 12:59

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