A new DNA-cloning vector for Haemophilus influenzae Rd

Joshi, V. P. ; Notani, N. K. (1983) A new DNA-cloning vector for Haemophilus influenzae Rd Journal of Biosciences, 5 (4). pp. 339-345. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/5/339-345.pdf

Related URL: http://dx.doi.org/10.1007/BF02716700


A new, high-efficiency, DNA-cloning vector pJ1-8 was derived in two steps from the chimeric plasmid pD7 consisting of RSF 0885(ampr) and Haemophilus influenzae chromosomal DNA. pJl-8 has only one EcoRI site and a molecular weight of only 2.5×106. No detectable ampr transformation was obtained with pJl-8 DNA. However, ampr transformation increases markedly if Haemophilus influenzae chromosomal DNA segments are spliced into it, providing a very facile assay for detecting inserts.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Cloning Vector; EcoRI Site; Efficient Insert Detector
ID Code:35208
Deposited On:11 Apr 2011 13:45
Last Modified:17 May 2016 18:07

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