Transformation of Haemophilus influenzae by plasmid RSF0885

Notani, Nihal K. ; Setlow, Jane K. ; McCarthy, David ; Clayton, Nancy-Lee (1981) Transformation of Haemophilus influenzae by plasmid RSF0885 Journal of Bacteriology, 148 (3). pp. 812-816. ISSN 0021-9193

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Plasmid RSF0885, which conferred ampicillin resistance, transformed competent Haemophilus influenzae cells with low efficiency (maximum, less than 0.01%). As judged by competition experiments and uptake of radioactivity, plasmid RSF0885 deoxyribonucleic acid was taken up into competent H. influenzae cells several orders of magnitude less efficiently than H. influenzae chromosomal deoxyribonucleic acid. Plasmid RSF0885 transformed cells with even lower efficiency than could be accounted for by the low uptake. Transformation was not affected by rec-1 and rec-2 mutations in the recipient, and strains cured of the plasmid did not show increased transformation. Plasmid molecules cut once with a restriction enzyme that made blunt ends did not transform. Transformation was favored by the closed circular form of the plasmid.

Item Type:Article
Source:Copyright of this article belongs to American Society for Microbiology.
ID Code:35195
Deposited On:11 Apr 2011 13:45
Last Modified:17 May 2016 18:06

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