A D-glucosylated form of dextransucrase: demonstration of partial reactions

Luzio, Gary A. ; Parnaik, Veena K. ; Mayer, Robert M. (1983) A D-glucosylated form of dextransucrase: demonstration of partial reactions Carbohydrate Research, 121 . pp. 269-278. ISSN 0008-6215

Full text not available from this repository.

Official URL: http://linkinghub.elsevier.com/retrieve/pii/000862...

Related URL: http://dx.doi.org/10.1016/0008-6215(83)84023-3


A D-glucosylated form of dextransucrase, whose preparation and characteristics have just been reported in Carbohydr. Res., was employed in a series of studies designed to explore the question of whether the bound sugars participate in the reactions catalyzed by the enzyme. When exposed to maltose, a good acceptor-substrate, monomeric D-glucosyl groups were rapidly transferred to the disaccharide, affording a trisaccharide. In the absence of an acceptor, monomeric D-glucose was released from the enzyme by hydrolysis. In a reaction with D-fructose, the charged enzyme catalyzed the formation of sucrose. Finally, in the presence of unlabeled sucrose, monomeric D-glucosyl groups were chased into enzyme-associated oligomers. Evidence is also presented which indicates that the various pathways for the bound D-glucosyl groups are competitive. The significance of these observations is discussed.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:34727
Deposited On:11 Apr 2011 14:00
Last Modified:11 Apr 2011 14:00

Repository Staff Only: item control page