Monohybrid and dihybrid segregations in the progenies of tobacco transformed for kanamycin resistance with a Ti-vector system

Viegas, Patricia ; Mathews, Helena ; Bhatia, C. R. ; Notani, N. K. (1987) Monohybrid and dihybrid segregations in the progenies of tobacco transformed for kanamycin resistance with a Ti-vector system Journal of Genetics, 66 (1). pp. 25-31. ISSN 0022-1333

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Official URL: http://www.ias.ac.in/jarch/jgenet/66/25.pdf

Related URL: http://dx.doi.org/10.1007/BF02934453

Abstract

A chimeric DNA construction having nopaline synthase promoter, coding sequences of neomycin phosphotransferase gene conferring resistance to antibiotic kanamycin and OCS (octopine synthase) polyadenylation sequences bracketed by T-DNA ends was transferred to tobacco. Leaf discs were infected with A. tumefaciens containing disarmed, cointegrate plasmid pGV3850:: 1103 and allowed to form a callus in the presence of kanamycin. Shoots regenerated from infected leaf discs either through the callus or arising directly were further selected for their ability to root in kanamycin-containing media. Among the nine transgenic plants that were progeny tested, the transferred bacterial gene segregated as monohybrid ratio (3 KanR: 1 KanS) in seven. Segregation data of two plant progenies indicated the presence of two independent loci of KanR DNA insertion (15 KanR: 1 KanS). Back-cross segregation data were consistent with the monohybrid or independent assortment of duplicate factors. Thus in the two cases, a minimum independent integration of two copies of T-DNA each with a KanR marker is inferred.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:A. tumefaciens (pTi KanR); Tobacco Leaf Disc Infection; Genetic Transformation; Vertical Transmission; Monohybrid and Dihybrid Segregations
ID Code:34161
Deposited On:25 Apr 2011 11:25
Last Modified:17 May 2016 17:04

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