In vitro electroporation of tobacco pollen

Mishra, K. P. ; Joshua, D. C. ; Bhatia, C. R. (1987) In vitro electroporation of tobacco pollen Plant Science, 52 (1-2). pp. 135-139. ISSN 0168-9452

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Tobacco pollen grains subjected in vitro to short electrical pulses of increasing intensities showed progressive decrease in germination and fluorescein diacetate staining. The ability of pollen to retain fluorescein was dependent on the field strength and the period of post pulse incubation. The decrease in number of fluorescing pollen with the field treatment was due to increase in membrane permeability. The field induced leaks in the membrane are reversible and appear to possess discriminating properties towards solutes. Permeabilisation by pulsed field may enable incorporation of macromolecules in viable pollen.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Electroporation; Pollen Germination; Membrane Permeability; Fluorescence
ID Code:33920
Deposited On:25 Apr 2011 11:25
Last Modified:25 Apr 2011 11:25

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