Transgenic plants of mustard Brassica juncea (L.) czern and coss

Mathews, Helena ; Bharathan, N. ; Litz, R. E. ; Narayanan, K. R. ; Rao, P. S. ; Bhatia, C. R. (1990) Transgenic plants of mustard Brassica juncea (L.) czern and coss Plant Science, 72 (2). pp. 245-252. ISSN 0168-9452

Full text not available from this repository.

Official URL:

Related URL:


The recovery of transgenic mustard plants from the R1 progeny of regenerated plants using cotyledon explants treated with Agrobacterium tumefaciens (C58C1, pGV3850::1103) is described. Adventitious shoots were isolated from cotyledonary callus treated with A. tumefaciens, and the shoot apices were sliced longitudinally and grown on selection medium. Regenerants were also grown on selection medium. After the second subculture, shoots with a higher ratio of green to white and/or purple were rooted. These were transplanted into soil and selfed. Seeds from R0 generation were germinated in vitro and shoot tip and cotyledon explants were screened for kanamycin resistance. Solid green shoots were obtained from cotyledon explants on selection medium containing 20 mg/l kanamycin. These were transplanted into soil and grown to maturity. Southern blot analysis of R1 plants showed intact integration of T-DNA into the Brassica juncea genome. This was further confirmed by seed germination and seedling growth on medium containing 200 mg/l kanamycin.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Brassica juncea; Transformation; Transgenic Plants
ID Code:33620
Deposited On:13 Feb 2012 07:41
Last Modified:13 Feb 2012 07:41

Repository Staff Only: item control page