Aspartokinase of a lysine producing mutant of Micrococcus glutamicus

Lakshman, Meena ; Shenoy, B. C. ; Raghavendra Rao, M. R. (1981) Aspartokinase of a lysine producing mutant of Micrococcus glutamicus Journal of Biosciences, 3 (1). pp. 57-67. ISSN 0250-5991

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Aspartokinase from Micrococcus glutamicus AEC RN-13-6/1 [a homoserine requiring, S-(2-aminoethyl)-L-cysteine resistant, lysine producing strain] was purified 71 fold. The partially purified enzyme was inhibited by L-lysine. L-threonine, L-methionine, L-isoleucine, L-valine and L-phenylalanine activated the enzyme and reversed the inhibition by L-lysine. Aspartokinase activity was not derepressed by growth-limiting concentrations of L-threonine and/or L-methionine. It was not repressed by an excess of L-lysine (20 mM) and/or L-isoleucine (15.3 mM). The degree of activation or inhibition by amino acids was dependant on the composition of the growth medium. This observation is in contrast with the enzyme from the original (non-lysine-producing) strain which was inhibited by lysine or threonine and in a concerted manner by threonine plus lysine.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Aspartokinase; Micrococcus Glutamicus
ID Code:33274
Deposited On:30 Mar 2011 13:17
Last Modified:17 May 2016 16:07

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