Effect of some metabolic inhibitors on rooting cuttings of Phaseolus mungo under varying light conditions and its relationship with auxin and nutrition

Abstract

Water and 1 mg1^-1 each of IAA and IBA completely inhibited on the cuttings of Phaseolus mungo obtained from the seedlings raised under far-red light but rooting took place in the dark and under white and red lights. Sucrose, however, caused rooting under far-red light and its effectiveness increased with the addition of IAA and IBA to the sucrose medium IBA being more effective. Culturing in 1 and 5 mg 1^-1 each of FudR, actinomycin-D, cycloheximide and chloramphenicol after pre-treatment with water, IAA, IBA, sucrose and IAA/IBA + sucrose inhibited rooting. The effect increased with the concentration of each inhibitor. In contrast to this, the culturing in water, IAA, IBA, sucrose and IAA/IBA + sucrose after pre-treatment with these metabolic inhibitors produced varying effects. While the inhibition persisted in water, IAA and IBA and even in 5 mg 1^-1 of each inhibitor that in sucrose stimulated rooting and the effect increased with the addition of IAA and IBA to sucrose, the effect of IBA + sucrose being more pronounced. This stimulation was irrespective of the inhibitor and light condition except that 1 mg 1^-1 actinomycin-D inhibited rooting in the dark and under far-red light.