Effect of temperature and some antimetabolites on the interaction effects of auxin and nutrition in rooting etiolated stem segments of Salix tetrasperma

Jain, M. K. ; Nanda, K. K. (1972) Effect of temperature and some antimetabolites on the interaction effects of auxin and nutrition in rooting etiolated stem segments of Salix tetrasperma Physiologia Plantarum, 27 (2). pp. 169-172. ISSN 0031-9317

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/j.1399-...

Related URL: http://dx.doi.org/10.1111/j.1399-3054.1972.tb03595.x

Abstract

Rooting on 2.5 cm long stem segments of Salix tetrasperma was optimal when cultured in 5.0 mg/I IAA in combination with 1.0 % glucose at 30°C, and in combination with 0.1 % glucose at 15°C. 5-Fluorodeoxyuridine (FUDR), actinomycin-D and cycloheximide all inhibited rooting and the inhibitory effect in each case increased with concentration. 5-Fluorouracil (FU), on the other hand, was ineffective. The results show that auxins probably act as a triggering agent for the synthesis of specific enzyme proteins that are required for the initiation of root primordia at the transcriptional level and that a correct balance of auxin and nutrition which may vary with the environmental conditions, is necessary for optimal rooting.

Item Type:Article
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