A saline medium for maintaining isolated heart of Pila globosa, Swainson

Lal, M. B. ; Agarwal, R. A. (1968) A saline medium for maintaining isolated heart of Pila globosa, Swainson Proceedings of the Indian Academy of Sciences, Section B, 67 (1). pp. 1-7. ISSN 0370-0097

PDF - Publisher Version

Official URL: http://www.ias.ac.in/jarch/procb/67/1-7.pdf

Related URL: http://dx.doi.org/10.1007/BF03051678


Some known standard salines for molluscs were found unsuitable for maintaining the heart of Pila in vitro. A new saline for Pila has been devised keeping in view the ionic composition, pH and osmotic pressure of blood of Pila. A saline having the composition of blood was not found suitable for maintaining isolated heart of Pila but reduction of percentage of potassium made it so. The osmotic pressure was adjusted with addition of glucose which also provides energy. Changes of pH between 5 and 8·5 did not have an effect on the heart-beat of Pila. It was suggested that a saline containing only the principal ions of blood was not suitable because other trace and organic substances present in the blood were perhaps playing an important role in the regular beating of the heart. Adjustment in the proportion of different ions and addition of glucose did partly compensate for the absence of these substances.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
ID Code:32769
Deposited On:29 Jun 2011 10:16
Last Modified:17 May 2016 15:37

Repository Staff Only: item control page