Interaction of amino acids with glycyl-glycine transport in the mammalian intestine

Ganapathy, V. ; Hellier, M. D. ; Radhakrishnan, A. N. (1979) Interaction of amino acids with glycyl-glycine transport in the mammalian intestine Journal of Biosciences, 1 (1). pp. 1-12. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/1/1-12.pdf

Related URL: http://dx.doi.org/10.1007/BF02702882

Abstract

In order to investigate a possible interaction between free amino acids and dipeptides during their mucosal uptake in man and monkey, perfusion studies in vivo and uptake studiesin vitro using labelled and non-labelled dipeptides and amino acids have been carried out. In contrast to the observations of other workers, inhibition of glycyl-glycine uptake was observed with free leucine and methioninc but not with glycine, proline, hydroxyproline or alanine. Leucine and methionine caused inhibition of cytosol glycyl-glycine hydrolase activity, while glycine had no effect. The dipeptide uptake and dipeptide hydrolysis by cytosol enzyme was competitively inhibited by leucine. Although brush border glycyl-glycine hydrolase was also inhibited by leucine, the inhibition was noncompetitive. These data indicate that a few free amino acids can interact with dipeptides during uptake. This interaction might occur either at the transport step or at the stage of intracellular dipeptide hydrolysis.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Intestine; Peptide Transport; Peptidases; Amino Acid Interaction
ID Code:32448
Deposited On:25 Apr 2011 12:46
Last Modified:17 May 2016 15:15

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