Lipoic acid and diabetes-Part III: Metabolic role of acetyl dihydrolipoic acid

Wagh, S. S. ; Gandhi, V. M. ; Natraj, C. V. ; Menon, K. K. G. (1986) Lipoic acid and diabetes-Part III: Metabolic role of acetyl dihydrolipoic acid Journal of Biosciences, 10 (2). pp. 171-179. ISSN 0250-5991

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Rat liver lipoyl transacetylase catalyzes the formation of acetyl dihydrolipoic acid from acetyl coenzyme A and dihydrolipoic acid. In an earlier paper the formation of acetyl dihydrolipoic from pyruvate and dihydrolipoic acid catalyzed by pyruvate dehydrogenase has been reported. Acetyl dihydrolipoic acid is a substrate for citrate synthase, acetyl coenzyme A carboxylase and fatty acid synthetase. The Vmax for citrate synthase with acetyl dihydrolipoic acid was identical to acetyl coenzyme A (approximately 1 μmol citrate formed/min/mg protein) while the apparent Km was approximately 4 times higher with acetyl dihydrolipoic acid as the substrate. This may be due to the fact that synthetic acetyl dihydrolipoic acid is a mixture of 4 possible isomers and only one of them may be the substrate for the enzymatic reaction. While dihydrolipoic acid can replace coenzyme A in the activation of succinate catalyzed by succinyl coenzyme A synthetase, the transfer of coenzyme A between succinate and acetoacetyl dihydrolipoic acid catalyzed by succinyl coenzyme A: 3 oxo-acid coenzyme A transferase does not occur.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Lipoic Acid; Acetyl Coenzyme A; Acetyl Dihydrolipoic Acid; Citrate Synthase
ID Code:31529
Deposited On:11 Mar 2011 09:53
Last Modified:17 May 2016 14:13

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